Recombinant Human Podoplanin/PDPN Protein (His Tag)
Size: 50μg
Catalogue Numbers: PKSH032909-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Podoplanin; PDPN
Target Synonym: Podoplanin; Aggrus; Glycoprotein 36; Gp36; PA2.26 Antigen; T1-Alpha; T1A; PDPN; GP36
UNIProt ID: Q86YL7
Research Areas: Tags & Cell Markers; Cardiovascular
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q86YL7
Background: Podoplanin is a type-1 transmembrane protein that belongs to Podoplanin family. PDPN expressed in various specialized cell types throughout the body. It highly expressed in placenta; lung; skeletal muscle and brain; weakly expressed in brain; kidney and liver. In placenta; PDPN expressed on the apical plasma membrane of endothelium; in lung; expressed in alveolar epithelium. PDPN physiological function is related to its mucin-type character. PDPN may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes; induces changes in cell morphology with transfected cells showing an elongated shape; numerous membrane protrusions; and major reorganization of the actin cytoskeleton; increased motility and decreased cell adhesion. It requires for normal lung cell proliferation and alveolus formation at birth and Induces platelet aggregation. Nevertheless; it doesn’t have any effect on amino acid transport and the aquaporin-type water channels.
Sequence: Ala23-Leu131
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 12.2 kDa
Observed MW: 27 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only