Recombinant Human POMGNT1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032917-10, PKSH032917-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: POMGNT1
Target Synonym: Protein O-Linked-Mannose Beta-1; 2-N-Acetylglucosaminyltransferase 1; POMGnT1; UDP-GlcNAc:Alpha-D-Mannoside Beta-1; 2-N-Acetylglucosaminyltransferase I.2; POMGNT1; MGAT1.2
UNIProt ID: Q8WZA1
Research Areas: Tags & Cell Markers
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q8WZA1
Background: Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).
Sequence: Leu59-Thr660
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 69.3 kDa
Observed MW: 74 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only