Recombinant Human PRCP Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032721-10, PKSH032721-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PRCP
Target Synonym: Lysosomal Pro-X Carboxypeptidase; Angiotensinase C; Lysosomal Carboxypeptidase C; Proline Carboxypeptidase; Prolylcarboxypeptidase; PRCP; PRCP; PCP
UNIProt ID: P42785
Research Areas: Cell biology; Cardiovascular
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P42785
Background: Lysosomal Pro-X Carboxypeptidase (PRCP) belongs to the peptidase S28 family. PRCP is detected in many tissues, with highest levels observed in placenta, lung, and liver. It is also present in the heart, brain, pancreas, and kidney. PRCP exists as a homodimer. PRCP cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. PRCP has been shown to be an activator of the cell matrix-associated prekallikrein.
Sequence: Leu22-His496
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM NaAc-HAc, 150mM NaCl, 10%Glycerol, pH4.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 54.5 kDa
Observed MW: 72 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only