Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030353-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PRKD2; PKD2
Target Symptom: HSPC187; nPKC-D2; PKD2
Research Area: Signal Transduction;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His & GST
Accession: NP_057541.2
Background: Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains onePH domain, twophorbol-ester/DAG-type zinc fingers and oneprotein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Activity: The specific activity was determined to be > 30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
Sequence: Met 1-Leu 878
Purity: > 82 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 124 kDa
Observed MW: 120 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only