Recombinant Human PSA/KLK3 Protein (aa 25-261, His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032949-10, PKSH032949-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PSA; KLK3
Target Synonym: APS; Hk3; KLK2A1; PSA; Prostate-Specific Antigen; PSA; Gamma-Seminoprotein; Seminin; Kallikrein-3; P-30 Antigen; Semenogelase;
UNIProt ID: P07288
Research Areas: Signal Transduction; Tags & Cell Markers; Neuroscience; Cancer; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P07288
Background: KLK3; also known as APS; is short for Prostate-specific antigen. It is a 261 aa. protein which belongs to the peptidase S1 family and Kallikrein subfamily. This protein has 5 isforms produced by alternative splicing. It is a secreted protein and can forms a heterodimer with SERPINA5 which can inhibit its activity. KLK3 is also strongly inhibited by Zn2+; 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins; which are avid zinc binders. KLK3 can hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Sequence: Ile25-Pro261
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl; 1mM CaCl2; pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 27.1 kDa
Observed MW: 35 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only