Recombinant Human PVRIG (C-mFc)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH034019-10, PKSH034019-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PVRIG
Target Symptom: C7orf15; CD112R; PVRIG; transmembrane protein PVRIG; C7orf15MGC138295; MGC104322; MGC138297; MGC2463
Research Area: Cell biology
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-mFc
UNIProt ID: Q6DKI7
Accession: Q6DKI7
Background: Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells. PVRIG may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
Activity: Immobilized Human PVRIG-mFc(Cat#PKSH034019) at 10μg/ml (100 μl/well) can bind Biotinylated Human Nectin-2-His-Avi(Cat#PKSH034032). The ED50 of Biotinylated Human Nectin-2-His-Avi(Cat#PKSH034032) is 0.42 ug/ml.
Sequence: Thr41-Asp171
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 40.1 kDa
Observed MW: 45-55 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only