Recombinant Human PYM1/WIBG Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032857-10, PKSH032857-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PYM1; WIBG
Target Synonym: Partner of Y14 and Mago; Protein Wibg Homolog; WIBG; PYM
UNIProt ID: Q9BRP8
Research Areas: epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: Q9BRP8
Background: Partner of Y14 and Mago (WIBG) is a key regulator of the Exon Junction Complex (EJC). EJC is a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs, is a positional landmarker for the intron exon structure of genes, and directs post-transcriptional processes in the cytoplasm, for instance mRNA export, nonsense-mediated mRNA decay or translation. WIBG is a cytoplasmic RNA-binding protein, it can be excluded from nucleus by Crm1. WIBG as a cooperateing partner of Mago-14, relates with Mago-14 by its N-terminal domain.
Sequence: Met 1-Leu204
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 10% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 23.7 kDa
Observed MW: 16-30 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only