Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031269-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: R-Spondin 1; RSPO1
Target Symptom: RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1
Research Area: Developmental Biology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture; ELISA
Fusion Tag: C-His
Accession: NP_001033722.1
Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Activity: 1. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml.
2. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear ranger of 6. 4-800 ng/ml.
3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Sequence: Met 1-Ala 263
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 28.2 kDa
Observed MW: 42 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only