Recombinant Human/Rhesus HER4/ErbB4 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031648-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Human; Rhesus HER4; ErbB4
Target Symptom: Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; ERBB4; HER4
Research Area: Signal Transduction; Tags & Cell Markers; Cancer; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_005226.1
Background: ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains; a transmembrane domain; a tyrosine kinase domain; a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain; heart; kidney; in addition to skeletal muscle; parathyroid; cerebellum; pituitary; spleen; testis and breast. And lower levels in thymus; lung; salivary gland; and pancreas. It specifically binds to and is activated by neuregulins; NRG-2; NRG-3; heparin-binding EGF-like growth factor; betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart; the central nervous system and the mammary gland; gene transcription; cell proliferation; differentiation; migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development; and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system; especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation; induction of milk proteins and lactation.
Activity: Immobilized human ErbB4-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 , The EC50 of biotinylated human NRG1 is 0.4-0.92 μg/ml.
Sequence: Met 1-Arg649
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 71.1 kDa
Observed MW: 102 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only