Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033002-10, PKSH033002-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ribonuclease 3; RNASE3
Target Synonym: Eosinophil Cationic Protein; ECP; Ribonuclease 3; RNase 3; RNASE3; ECP; RNS3
UNIProt ID: P12724
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P12724
Background: Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Sequence: Arg28-Ile160
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mMDTT, 10%Glycerol, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.6 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only