Recombinant Human Ribonuclease A/RNASE1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033004-10, PKSH033004-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ribonuclease A; RNASE1
Target Synonym: Ribonuclease Pancreatic; HP-Rnase; RIB-1; RNase UpI-1; Ribonuclease 1; RNase 1; Ribonuclease A; RNase A; RNASE1; RIB1; RNS1
UNIProt ID: P07998
Research Areas: epigenetics and nuclear signal
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P07998
Background: Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly(C) as a substrate and hydrolyzes 2', 3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA.
Sequence: Lys29-Thr156
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 55.0 kDa
Observed MW: 21-28 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only