Recombinant Human Rock-2 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100162-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Rock-2
Target Synonym: Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; ROCK 2; Rock II; Rock2; ROCK2; Rock2m; ROK alpha; ROKalpha
UNIProt ID: O75116
Research Areas: Cancer; Cell Biology; Signal Transduction
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: O75116
Background: ROCK2/II (Rho-associated protein kinase 2; also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92-354), a coiled-coil region (aa 429-1131) and a zinc-finger region (aa 1260-1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids.
Sequence: Leu 904-Gly 1140
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 25.96 kDa
Observed MW: 35 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only