Recombinant Human S100A8&S100A9 Heterodimer Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033381-10, PKSH033381-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: S100A8&S100A9 Heterodimer
Target Symptom: S100A8/S100A9 Heterodimer
Research Area: Signal Transduction; Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; immunology
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
UNIProt ID: P05109&P06702
Accession: P05109&P06702
Background: S100A8 is a member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. Calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.
Sequence: Met 1-Glu93(S100A8)&Thr 2-Pro114(S100A9)
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM HEPES, 10%Glycerol, 150mM NaCl, 2.5mM EDTA, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 11.7&13.2 kDa
Observed MW: 15 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only