Recombinant Human SERPINB1/PI2 Protein (Human Cells, His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032695-10, PKSH032695-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SERPINB1; PI2
Target Synonym: Leukocyte elastase inhibitor; SERPINB1; Monocyte/neutrophil elastase inhibitor; M/NEI; Peptidase inhibitor 2; PI-2; PI2; EI; ELANH2; HEL-S-27; HEL57; LEI; MNEI;
UNIProt ID: P30740
Research Areas: Cell biology; Cardiovascular
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P30740
Background: SERPINB1 is a member of the serpin family and Ov-serpin subfamily. As protease inhibitors; serpins have an array of functions including regulating blood coagulation; fibrinolysis; the complement pathway; angiogenesis; inflammation; tumor suppression; extracellular matrix remodeling; and cell motility. SERPINB1 regulates the activity of the neutrophil proteases elastase; cathepsin G; proteinase-3; chymase; chymotrypsin; and kallikrein-3. Reactive bond 1 of SerpinB1 is specific for reaction with chymotrypsin-like protease such as cathepsin G; chymotrypsin or chymase. Reactive bond 2 of SerpinB1 is specific for reaction with elastase-like protease such as neutrophyl elastase; proteinase-3; pancreatic elastase or PSA. In addition; SERPINB1 also functions as a potent intracellular inhibitor of granzyme H.
Sequence: Met 1-Pro379
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 43.8 kDa
Observed MW: 44 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only