Recombinant Human Siglec-7/CD328 (C-6His)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033850-10, PKSH033850-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Siglec-7; CD328
Target Symptom: Sialic acid-binding Ig-like lectin 9; Siglec-9; CDw329; Protein FOAP-9; SIGLEC9
Research Area: Cardiovascular; Neuroscience; immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
UNIProt ID: Q9Y286
Accession: Q9Y286
Background: Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes. It binds equally well to both alpha 2, 3- and alpha 2, 6-linked sialic acid. The gene encoding Siglec-7 was mapped to chromosome 19q13.3.
Sequence: Gln19-Leu353
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 37.8 kDa
Observed MW: 50-70 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only