Recombinant Human Signal Transducer CD24/CD24 (C-mFC)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033949-10, PKSH033949-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD24
Target Symptom: Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; CD24; CD24A
Research Area: Neuroscience; Cancer; immunology; metabolism; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-mFc
UNIProt ID: P25063
Accession: P25063
Background: Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.
Activity: Immobilized Human CD24-mFc at 2μg/ml (100 μl/well) can bind Anti-Human CD24 mAb. The ED50 of Anti-Human CD24 mAb is 10.1 ng/ml.
Sequence: Ser27-Gly59
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 29.5 kDa
Observed MW: 40-60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only