Recombinant Human Sonic Hedgehog/SHH Protein (Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033519-10, PKSH033519-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Sonic Hedgehog; SHH
Target Symptom: Sonic Hedgehog Protein; SHH; HHG-1; SHH
Research Area: Cancer; Developmental Biology; epigenetics and nuclear signal; metabolism; Stem cells
Target Species: Human
Expression Host: E.coli
Application: ELISA
Fusion Tag: None
UNIProt ID: Q15465
Accession: Q15465
Background: Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems; such as limbs and brain. SHH also plays an important role in adult; including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor; and undergoes a series of processing during secretion. After the removal of the signal peptide; a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface; which releases the repression of the activity of Smoothened (Smo); a G-protein coupled receptor; by Ptc.
Activity: Immobilized Human SHH at 1μg/ml(100 μl/well) can bind Human BOC-His(Cat: PKSH032123).
Sequence: Cys24-Gly197
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19.69 kDa
Observed MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only