Recombinant Human ST8SIA1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033251-10, PKSH033251-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ST8SIA1
Target Symptom: Alpha-N-Acetylneuraminide Alpha-2; 8-Sialyltransferase; Alpha-2; 8-Sialyltransferase 8A; Ganglioside GD3 Synthase; Ganglioside GT3 Synthase; Sialyltransferase 8A; SIAT8-A; Sialytransferase St8Sia I; ST8SiaI; ST8SIA1; SIAT8; SIAT8A
Research Area: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
UNIProt ID: Q92185
Accession: Q92185
Background: α-N-Acetylneuraminide α-2, 8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus.
Sequence: Tyr49-Ser356
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 36.2 kDa
Observed MW: 48 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only