Recombinant Human SULT1B1 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031074-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SULT1B1
Target Symptom: Sulfotransferase Family Cytosolic 1B Member 1; ST1B1; Sulfotransferase 1B1; Sulfotransferase 1B2; ST1B2; Thyroid Hormone Sulfotransferase; SULT1B1; ST1B2; SULT1B2
Research Area: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Application: Enzyme
Fusion Tag: N-His
Accession: NP_055280.2
Background: Sulfotransferase family cytosolic 1B member 1; also known as Sulfotransferase 1B1; Sulfotransferase 1B2; Thyroid hormone sulfotransferase; SULT1B1 and ST1B2; is a cytoplasm protein which belongs to thesulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones; neurotransmitters; drugs; and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1B1 is highly expressed in the liver; peripheral blood leukocytes; colon (mucosal lining); small intestine (jejunum) and spleen. A lesser expression of SULT1B1 was observed in the lung; placenta and thymus. SULT1B1 catalyzes the sulfate conjugation of many hormones; neurotransmitters; drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds; and therefore their renal excretion; but it can also result in bioactivation to form active metabolites. SULT1B1 sulfates dopamine; small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones; including 3; 3'-diiodothyronine; triidothyronine; reverse triiodothyronine and thyroxine.
Activity: Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is > 40 pmoles/min/μg.
Sequence: Leu 2-Ile 296
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 0.1 M NaCl, 10% glycerol, 1mM DTT, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 35.7 kDa
Observed MW: 34 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only