Recombinant Human TCN2 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031522-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: TCN2
Target Symptom: Transcobalamin-II; D22S676; D22S750; TC-2; TC2; TCII
Research Area: Signal Transduction; Cardiovascular; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_000346.2
Background: Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.
Activity: Immobilized human TCN2-His at 10μg/mL (100μL/well) can bind biotinylated mouse CD320-His. The EC50 of biotinylated mouse CD320-His is 18-42 ng/mL.
Sequence: Met 1-Trp 427
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 46.7 kDa
Observed MW: 43 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only