Recombinant Human TGFB1/TGF-beta 1 Protein (Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032007-10, PKSH032007-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: TGFB1; TGF-beta 1
Target Symptom: Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB; CED; DPD1; TGF-beta 1
Research Area: Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism; Stem cells
Target Species: Human
Expression Host: CHO Stable Cells
Application: Cell culture
Fusion Tag: None
UNIProt ID: P01137
Accession: P01137
Background: Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone; articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions; including the control of cell growth; cell proliferation; cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.
Activity: Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/ml
Sequence: Ala279-Ser390
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Glycine, 150mM NaCl, pH2.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Calculated MW: 12.8 kDa
Observed MW: 13 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only