Recombinant Human Thrombopoietin/TPO Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033110-10, PKSH033110-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Thrombopoietin; TPO
Target Symptom: Thrombopoietin; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Myeloproliferative leukemia virus oncogene ligand; THPO
Research Area: Cell biology; Cardiovascular; Cancer; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: N-His & C-His
UNIProt ID: P40225
Accession: P40225
Background: Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Activity: Measured in a cell proliferation assay using MO7E human megakaryocytic leukemic cells. The ED50 for this effect is 0.55 ng/ml.
Sequence: Ser22-Gly353
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 37.3 kDa
Observed MW: 70-90 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only