Recombinant Human TLK1/PKU-beta Protein (His & GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030339-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: TLK1; PKU-beta
Target Symptom: KIAA0137; PKU-beta
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His & GST
UNIProt ID: Q9UKI8
Accession: Q9UKI8-1
Background: The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.
Activity: The specific activity was determined to be 1. 6 nmol/min/mg using Histone H3 protein as substrate.
Sequence: Met 1-Tyr 766
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 114 kDa
Observed MW: 125 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only