Recombinant Human TNFSF4/OX40L Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032842-10, PKSH032842-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: TNFSF4; OX40L
Target Symptom: Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFSF4; CD252; TXGP1; CD134L; CD252; GP34; OX-40; TXGP1
Research Area: immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: N-6His
UNIProt ID: P23510
Accession: P23510
Background: Tumor necrosis factor ligand superfamily member 4(TNFSF4/OX40L) is a single-pass type II membrane protein.OX40L is expressed on the surface of activated B cells; T cells; dendritic cells and endothelial cells. OX40L binds to OX40 (CD134); a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40-OX40L co-stimulates signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. It involved in T-cell proliferation and cytokine production. Additional; it has been found association with systemic lupus erythematosus; no association with occurrence of atherosclerosis.
Activity: Immobilized Human OX40-Fc(Cat: PKSH032844) at 2μg/ml(100 μl/well) can bind Biotinylated Human OX40L-His. The ED50 of Human OX40L-His is 4. 7 ug/ml.
Sequence: Gln51-Leu183
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.3 kDa
Observed MW: 25-28 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only