Recombinant Human VAPB Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033405-10, PKSH033405-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: VAPB
Target Symptom: Vesicle-associated membrane protein-associated protein B/C; VAMP-B/VAMP-C; VAMP-associated protein B/C; VAP-B/VAP-C
Target Species: Human
Expression Host: E.coli
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: O95292
Accession: O95292-1
Background: vesicle-associated membrane protein-associated protein B/C (VAPB) is presents in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane. It can form homodimer; and heterodimer with VAPA. It also interacts with VAMP1; VAMP2; HCV NS5A; NS5B; ZFYVE27 and RMDN3. VAPB participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. It is involved in cellular calcium homeostasis regulation.
Activity: Immobilized Human EphB2-Fc(Cat: PKSH032012) at 1μg/ml(100 μl/well) can bind Human VAMPB-His. The ED50 of Human VAMPB-His is 5. 179ug/ml.
Sequence: Ala2-Pro132
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.0 kDa
Observed MW: 16 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only