Recombinant Human VCL/Vinculin Protein (His Tag)
Size: 50μg
Catalogue Number: PKSH031929-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: VCL, Vinculin
Target Synonym: Vinculin, Metavinculin, VCL, CMD1W, CMH15, HEL114, MV, MVCL
UNIProt ID: P18206
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P18206-2
Background: Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.
Sequence: Met 1-Gln 1066
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 118 kDa
Observed MW: 115 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only