Recombinant Human VDR/NR1I1 Protein (His Tag)
Size: 50μg
Catalogue Numbers: PKSH030931-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: VDR; NR1I1
Target Synonym: NR1I1; PPP1R163
UNIProt ID: P11473
Research Areas: Signal Transduction; Cancer; epigenetics and nuclear signal;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: C-His
Accession: P11473
Background: VDR (vitamin D(1, 25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1, 25(OH)2D3) and induce expression of the 1, 25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.
Sequence: Met 1-Ser 427
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50 kDa
Observed MW: 50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only