Recombinant Human VEGF-A/VEGF121 Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH033207-20, PKSH033207-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: VEGF-A; VEGF121
Target Symptom: Vascular endothelial growth factor A; VEGFA; VEGF-A; Vascular permeability factor; VPF; VEGF; VEGF121
Research Area: Signal Transduction; Cardiovascular; Cancer; Developmental Biology; metabolism;
Target Species: Human
Expression Host: E.coli
Application: ELISA
Fusion Tag: None
UNIProt ID: P15692
Accession: P15692
Background: Human VEGF121; also known as Vascular endothelial growth factor A; VEGFA; Vascular permeability factor; VPF and VEGF; is a homodimeric; heparin-binding glycoprotein which belongs to the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family. VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis; permeabilization of blood vessels and endothelial cell growth; increasing microvascular permeability; promoting cell migration and inhibiting apoptosis. Alternatively spliced transcript variants of VEGF-A encod either secreted or cell-associated isoforms. The lymphangiogenesis may be promoted by upregulation of VEGF121; which may in turn act in part via induction of VEGF-C. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors; heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways; does not activate angiogenesis and inhibits tumor growth.
Activity: Immobilized Human VEGF121 at 2μg/ml(100 μl/well) can bind Human VEGFR1-Fc(Cat: PKSH033445). The ED50 of Human VEGF121 is 0.08μg/mL.
Sequence: Ala27-Arg147
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 14.2 kDa
Observed MW: 16 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only