Recombinant Human WNT3A protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100436-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: WNT3A
Target Synonym: Protein Wnt-3a; WNT3A
UNIProt ID: P56704
Research Areas: Developmental Biology;
Neuroscience; Stem Cells
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P56704
Background: Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels. As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes.
Sequence: Ser19-Lys325
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 33.66 kDa
Observed MW: 38 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only