Recombinant Human XRCC5 & XRCC6 Heterodimer Protein
Size: 50μg
Catalogue Numbers: PKSH030480-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: XRCC5 & XRCC6 Heterodimer
Target Synonym: KARP-1; KARP1; KU80; Ku86; KUB2; NFIV
UNIProt ID: P13010&P12956
Research Areas: Cancer; epigenetics and nuclear signal
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-His
Accession: P13010&P12956
Background: X-ray repair cross-complementing protein 5, also known as 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, DNA repair protein XRCC5, Lupus Ku autoantigen protein p86, TLAA and XRCC5, is a nucleus and chromosome which belongs to theku80 family. XRCC5 is a single stranded DNA-dependent ATP-dependent helicase. XRCC5 has a role in chromosome translocation. X-ray repair cross-complementing protein 6, also known as 5'-deoxyribose-5-phosphate lyase Ku70, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, CTC box-binding factor 75 kDa subunit, Lupus Ku autoantigen protein p70, Thyroid-lupus autoantigen and XRCC6, is a nucleus and chromosome which belongs to theku70 family. Heterodimer of a XRCC6 and a XRCC5 subunit associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression.
Sequence: Met 1-Ile732&Met 1-Asp609
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 157 (85 + 72) kDa
Observed MW: 70 & 85 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only