Recombinant Human YY1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033137-10, PKSH033137-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: YY1
Target Synonym: Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; INO80S
UNIProt ID: P25490
Research Areas: Neuroscience; epigenetics and nuclear signal; Isotypeloading control
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: P25490
Background: Transcriptional repressor protein YY1（YY1）contains 4 C2H2-type zinc fingers and belongs to the YY transcription factor family. Multifunctional transcription factor exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. The effect on transcription regulation of the protein is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.
Sequence: Val221-Gly321
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 12.6 kDa
Observed MW: 17 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only