Recombinant Human ZWINT Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033243-10, PKSH033243-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: ZWINT
Target Symptom: ZW10 Interactor; ZW10-Interacting Protein 1; Zwint-1; ZWINT
Research Area: Cell biology; epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
UNIProt ID: O95229
Accession: O95229
Background: ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Sequence: Met 1-Pro277
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33.4 kDa
Observed MW: 36 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only