Recombinant Mouse BLMH/BLM Hydrolase Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040554-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: BLMH; BLM Hydrolase
Target Symptom: AI035728; Bh; Bmh
Target Species: Mouse
Expression Host: E.coli
Application: Enzyme
Fusion Tag: N-His
Accession: NP_848760.1
Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
Activity: Measured by its ability to hydrolyze Met-AMC. The specific activity is > 500 pmoles/min/μg.
Sequence: Asn 2-Glu 455
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 53.3 kDa
Observed MW: 47 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only