Recombinant Mouse Butyrylcholinesterase/BCHE Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040691-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Butyrylcholinesterase; BCHE
Target Symptom: C730038G20Rik
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
Accession: NP_033868.3
Background: Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as " pseudo" or " non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.
Activity: Measured by its ability to cleave Butyrylthiocholine. The specific activity is > 70 nmol/min/μg.
Sequence: Met 1-Leu 603
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 25mM Tris, 100mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 66.7 kDa
Observed MW: 60-70 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only