Recombinant Mouse Caspase-8/CASP8 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEM100044-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Caspase-8; CASP8
Target Synonym: CASP8_MOUSE; Caspase-8; Casp8; EC:3.4.22.61
UNIProt ID: O89110
Research Areas: Cell biology
Target Species: Mouse
Expression Host: E.coli
Fusion Tag: N-His
Accession: O89110
Background: Caspase-8 (Cysteine-aspartic acid protease 8/Casp8a; also named MCH5, FLICA and MACH alpha 1) is a 28 kDa member of the peptidase C14A family of enzymes. It is widely expressed and is considered an initiating caspase for the apoptotic cascade. Caspase-8 acts on a wide variety of substrates, including procaspases 3, 4, 6, 7, 9 and 10, c-FLIPL and procaspase-8 itself. Human procaspase-8a is a 54-56 kDa, 479 amino acid (aa) protein. It contains two N-terminal death domains (aa 1-177), followed by a catalytic site that utilizes His317Gly318 plus Cys360. Normally, it is an inactive, cytosolic monomer. But following death-domain (DD) containing receptor oligomerization, Caspase-8 is recruited to the death-inducing signaling complex (DISC) that forms around the death domains of the oligomerized receptor.
Sequence: Ser 219-Gly 376
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 17.27 kDa
Observed MW: 20 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only