Recombinant Mouse CD80/B7-1 Protein (Fc Tag)(Active)
Size: 100μg
Catalogue Number: PKSM040680-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD80; B7-1
Target Symptom: T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80; Cd28l; Ly-53; Ly53; MIC17; TSA1
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-hFc
UNIProt ID: Q00609
Accession: Q00609-1
Background: The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Activity: Immobilized human CTLA4 at 10 μg/mL (100 μl/well) can bind mouse CD80-Fc, The EC50 of mouse CD80-Fc is 28 ng/mL.
Sequence: Met 1-Lys 245
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50.7 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only