Recombinant Mouse EGFR Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSM040365-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: EGFR
Target Symptom: 9030024J15Rik; AI552599; Erbb; Errb1; Errp; wa-2; wa2; Wa5
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
UNIProt ID: Q01279
Accession: Q01279
Background: As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α , betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Activity: 1. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc, The EC50 of human EGF-Fc is 60-90 ng/mL.
2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc, The EC50 of mouse EGF-Fc is 70-100 ng/mL.
Sequence: Met 1-Ser 647
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 71 kDa
Observed MW: 100 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only