Recombinant Mouse Ephrin-A5/EFNA5 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041010-10, PKSM041010-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Ephrin-A5; EFNA5
Target Symptom: Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; Epl7; Eplg7; Lerk7; Efna5;
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: O08543
Accession: O08543
Background: Ephrin-A5 is a glycosylphosphatidylinositol (GPI)-anchored protein of the ephrin-A subclass of ephrin ligands that binds to the EphA subclass of Eph receptors. Ephrin-A5 has also been shown to bind to the EphB2 receptor. It is crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin-A5 binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Activity: Immobilized Human EphA8-Fc(Cat: PKSH032386) at 1. 5μg/ml(100 μl/well) can bind Human EFNA5-His. The ED50 of EFNA5-His is 12.63ug/ml.
Sequence: Gln21-Gln206
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 22.5 kDa
Observed MW: 25-28 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only