Recombinant Mouse GITR/TNFRSF18 Protein (Fc & His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041029-10, PKSM041029-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: GITR; TNFRSF18
Target Symptom: Tumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; Tnfrsf18; AITR; CD357; GITR-D; TNFRSF18
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc-6His
UNIProt ID: O35714
Accession: O35714
Background: Tumor necrosis factor receptor superfamily member 18(Gitr) contains 3 TNFR-Cys repeats and it have four isforms.IsformA、isformB and isformC is single-pass type I membrane protein and isformD is a secreted protein. The protein is the receptor for TNFSF18.It seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. It mediated NF-kappa-B activation via the TRAF2/NIK pathway.It binds to TRAF1, TRAF2, and TRAF3, but not TRAF5 and TRAF6 and binds through its C-terminus to SIVA1/SIVA.It preferentially expressed in activated T lymphocytes and up-regulated in peripherical mononuclear cells after antigen stimulation/lymphocyte activation.
Activity: Immobilized Human GITRL-His(Cat: PKSH032485) at 10μg/ml(100 μl/well) can bind Mouse GITR -Fc-His.
Sequence: Ser22-His153
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 41.12 kDa
Observed MW: 60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only