Recombinant Mouse Granzyme B(G,H)/GZMB Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041035-10, PKSM041035-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Granzyme B; GZMB
Target Synonym: Granzyme B(G,H); CTLA-1; Cytotoxic cell protease 1; CCP1; Fragmentin-2; Gzmb; Ctla-1; Ctla1
UNIProt ID: P04187
Target Species: Mouse
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: P04187
Background: Granzyme B(GZMB) contains 1 peptidase S1 domain and belongs to the peptidase S1 family. This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp and seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. The protein cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence: Gly19-Ser247
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.5 kDa
Observed MW: 35 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only