Recombinant Mouse HVEM/TNFRSF14 Protein (Fc Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040955-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: HVEM; TNFRSF14
Target Symptom: Tnfrsf14; Herpesvirus entry mediator; HVEM; TR2; TNF receptor-like molecule; ATAR; another TRAF-associated receptor; Tumor necrosis factor receptor superfamily member 14; Atar; HveA
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-Fc
Accession: NP_849262.1
Background: Mouse Protein Tnfrsf14, is a type I transmembrane protein belonging to the TNF receptor superfamily. It is tumor necrosis factor receptor superfamily member 14 and expressed on the surface of T cells during the resting state. Interaction of HVEM with TNF family member LIGHT co-stimulates T cells and promotes inflammation. HVEM also triggers inhibitory signaling cascade in effector T (Teff) cells and regulatory T cells (Tregs) as a ligand of B and T lymphocyte attenuator. Tnfrsf14 is detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. It has demonstrated that HVEM Ig is able to exert a significant antiviral effect against HSV-1 infection in vivo.
Activity: Immobilized Mouse HVEM-Fc at 6μg/ml(100 μl/well) can bind Human BTLA-His(Cat: PKSH033758). The ED50 of Mouse HVEM-Fc is 1. 17ug/ml.
Sequence: Gln39-Val207
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.6 kDa
Observed MW: 50&60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only