Recombinant Mouse M-CSF protein(N-His)(active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSM041498-20, PKSM041498-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: M-CSF
Target Symptom: TNFSF4; Ath; Ath-; Ath-1; Ath1; CD134; CD134L; OX-40L; OX4; TXGP1; Tnlg2b; Txgp; Txgp1l; gp3; gp34
Target Species: Mouse
Expression Host: E.coli
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: P07141
Accession: P07141
Background: Macrophage colony-stimulating factor 1（M-csf）is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
Activity: Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 2 ng/mL. The specific activity of recombinant mouse M-CSF is approximately > 5 x 105IU/mg.
Sequence: Lys32-Pro187
Purity: > 98 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only