Recombinant Mouse PGF/PIGF Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM041122-10, PKSM041122-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PGF; PIGF
Target Symptom: Placenta growth factor; PlGF; Plgf; D12S1900; PGFL; PLGF; PlGF-2; SHGC-10760
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-6His
UNIProt ID: P49764
Accession: P49764
Background: Placental growth factor is a protein that in humans is encoded by the PGF gene. It is a secreted protein and belongs to the PDGF/VEGF growth factor family. The protein is a member of the VEGF (vascular endothelial growth factor) sub-family-a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast.
Activity: Immobilized Human VEGFR1-Fc(Cat: PKSH033445) at 2μg/ml(100 μl/well) can bind Mouse PGF-His. The ED50 of Mouse PGF-His is 2. 85μg/mL.
Sequence: Val19-Pro158
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.9 kDa
Observed MW: 30 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only