Recombinant Mouse Pleiotrophin/PTN/HB-GAM Protein (His Tag)
Size: 50μg
Catalogue Numbers: PKSM041292-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Pleiotrophin; PTN; HB-GAM
Target Synonym: Pleiotrophin; PTN; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Osteoblast-specific factor 1; OSF-1; HARP; HB-GAM; HBBN; HBNF; OSF; Osf-1; Osf1
UNIProt ID: P63089
Target Species: Mouse
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P63089
Background: Pleiotrophin (PTN) is an secreted, strongly heparinbinding, developmentally regulated cytokine. PTN and midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family. PTN is a highly conserved protein human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity orgreater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis. PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates. Increased expression of PTN is correlated withneuronal development or stresses such as brain ischemia and Parkinson’s disease.
Sequence: Gly33-Asp168
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 16.1 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only