Recombinant Mouse SRC Kinase/c-SRC Protein (His & GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040305-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SRC Kinase; c-SRC
Target Symptom: AW259666; pp60c-src
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: ELISA
Fusion Tag: N-His-GST
Accession: NP_001020566.111
Background: Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.
Activity: 1. The specific activity was determined to be > 80 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
2. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.
Sequence: Met 1-Leu 535
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 87.7 kDa
Observed MW: 80 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only