Recombinant Rat AIF-M1 protein (His tag)
Size: 100μg
Catalogue Numbers: PDER100061-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: AIF-M1
Target Synonym: AIFM1; Apoptosis inducing factor 1; mitochondrial; Apoptosis inducing factor; CMTX4; COWCK; COXPD6; Harlequin; mitochondrial; PDCD 8; Programmed cell death 8; Striatal apoptosis inducing factor
UNIProt ID: Q9JM53
Research Areas: Cell Biology; Epigenetics and Nuclear Signaling; Cancer; Metabolism; Neuroscience
Target Species: Rat
Expression Host: E.coli
Fusion Tag: N-His
Accession: Q9JM53
Background: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Sequence: Arg 150-Val 299
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 16.39 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only