Recombinant Rat AST protein (His tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMR100007-20, PDMR100007-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: AST
Target Synonym: Aspartate aminotransferase; cytoplasmic; cAspAT; Cysteine aminotransferase; cytoplasmic; Cysteine transaminase; cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A; Got1
UNIProt ID: P13221
Target Species: Rat
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P13221
Background: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Sequence: Met1-Gln413
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 45.32 kDa
Observed MW: 45 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only