Recombinant Rat IL-6/Interleukin-6 Protein (Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSR030420-20, PKSR030420-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-6; Interleukin-6
Target Symptom: IL6
Target Species: Rat
Expression Host: E.coli
Application: Cell culture
Fusion Tag: None
Accession: NP_036721.1
Background: Interleukin-6 (IL-6) is a multifunctional α -helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Activity: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-6 ng/ml.
Sequence: Phe25-Thr211
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 40% acetonitrile, 0.1% TFA
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 21.9 kDa
Observed MW: 22 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only