Recombinant S. cerevisiae TIM16 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSQ050084-10, PKSQ050084-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: TIM16
Target Synonym: Mitochondrial import inner membrane translocase subunit TIM16; Presequence translocated-associated motor subunit PAM16; PAM16; TIM16
UNIProt ID: P42949
Target Species: S. cerevisiae
Expression Host: E.coli
Fusion Tag: None
Accession: P42949
Background: Mitochondrial import inner membrane translocase subunit TIM16 (TIM16) is an ssential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM16 is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. TIM16 may act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.
Sequence: Thr54-Ala119
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 7.9 kDa
Observed MW: 11 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only