Recombinant SARS-CoV-2 NSP12 protein
Sizes: 50μg, 100μg
Catalogue Numbers: PKSV030327-50, PKSV030327-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: NSP12
Target Synonym: SARS-CoV 2 nsp12; RNA-directed RNA polymerase; RdRp
UNIProt ID: P0DTC2
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: N-Gst
Accession: QHD43415.1
Background: The RdRp of SARS-CoV-2 is composed of a catalytic subunit known as nsp12 as well as two accessory subunits, nsp8 and nsp7. The structure of this RdRp has recently been reported; it is highly similar to the RdRp of SARS-CoV, a zoonotic coronavirus that spread into the human population in 2002. The nsp12 subunit contains an N-terminal nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain, an interface domain and a C-terminal RdRp domain. The RdRp domain resembles a right hand, comprising the fingers, palm and thumb subdomains that are found in all single-subunit polymerases. Subunits nsp7 and nsp8 bind to the thumb, and an additional copy of nsp8 binds to the fingers domain. Structural information is also available for nsp8–nsp7 complexes.
Sequence: Asp4891-Val5212
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 64 kDa
Observed MW: 60 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only